Composite

Part:BBa_K4609071:Design

Designed by: Yisong Wen   Group: iGEM23_JLU-China   (2023-10-09)


pET21a（+）-HSP 27


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NO


Source

All of our genetic sequences come from NCBI and are synthesized through PPL, a company with which we have deep cooperation.

References

1. Williams DC, Van Frank RM,Muth WL, et al.Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins[J].Science,1982,215(4533):687-9. 2. Freedman RB,Wetzel R.Protein engineering[J].Current Opinion in Biotechrology,1992,3(4)322 3. GlickBR.Metabolic load and heterologous gene expression[J].Biotechnol Adv, 1995,13(2):247-61. 4. Fatemeh Sadat Shariati, Malihe Keramati, Vahideh Valizadeh, et al. Comparison of E. coli based self-inducible expression systems containing different human heat shock proteins. ScientificReports. 11, 4576(2021).